A New Role for the Tumour Suppressor Lin-35 during Meiotic Recombination in Caenorhabditis Elegans

..........................................................................................................................ii
 TABLE OF CONTENTS........................................................................................................ iii
 LIST OF TABLES ..................................................................................................................v
 LIST OF FIGURES................................................................................................................vi
 LIST OF ABBREVIATIONS ..................................................................................................vii
 ACKNOWLEDGEMENTS ................................................................................................... viii
 CHAPTER 1: INTRODUCTION ..............................................................................................1
 1.1 The role of meiotic recombination ...........................................................................1
 1.2 Meiotic prophase and recombination in Caenorhabditis elegans ............................1
 1.3 Rec-1 randomizes crossover distribution..................................................................6
 1.4 The role of chromatin structure and chromatin remodeling during meiotic recombination .................................................................................................................8
 1.5 The tumour suppressor and chromatin modifier LIN-35........................................10
 1.6 lin-35 (n745) ...........................................................................................................12
 1.7 Thesis objectives.....................................................................................................12
 CHAPTER 2: MATERIALS AND METHODS.........................................................................13
 2.1 General methods .....................................................................................................13
 2.2 Strain construction ..................................................................................................14
 2.3 Crosses ....................................................................................................................16
 2.4 Calculating recombination frequency.....................................................................16
 2.5 Brood analysis ........................................................................................................17
 2.6 RNAi.......................................................................................................................17